The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Role of Calcium in Tumor Necrosis Factor-α Production by Activated Macrophages
Naoko WatanabeJunko SuzukiYoshiro Kobayashi
Author information
JOURNAL FREE ACCESS

1996 Volume 120 Issue 6 Pages 1190-1195

Details
Abstract

The role of calcium in the production of tumor necrosis factor-α (TNF-α) by the lipopolysaccharide (LPS)-stimulated macrophage cell line, J 774.1, was investigated. Flow cytometric measurement of intracellular calcium concentration ([Ca]1) using 2-(3, 6-bis (acetyloxy)-2, 7-dichloro-9H-xanthen-9-yl) benzoic acid (fluo-3)-loaded J 774.1 cells revealed that LPS at more than 0.1 μg/ml increased [Ca]1 transiently in the presence or absence of serum, and that a mixture of a calcium chelator, ethyleneglycol-bis(β-aminoethyl ether)-N, N, N', N'-tetraacetic acid (EGTA), and a calcium release blocker from intracellular store sites, 8-(diethylamino)-octyl-3, 4, 5-trimethoxybenzoate hydrochloride (TMB-8), inhibited the [Ca]1 response induced by LPS. In concordance with this, production of TNF-α was inhibited by EGTA and/or TMB-8. These reagents also reduced the level of TNF-α mRNA significantly. These results indicate that the transient increase of [Ca]1 plays a role in LPS-induced expression of TNF-α by the macrophage cell line.

Content from these authors
© The Japanese Biochemical Society
Previous article Next article
feedback
Top