The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
CYP51-Like Gene of Mycobacterium tuberculosis Actually Encodes a P450 Similar to Eukaryotic CYP51
Yuri AoyamaTadao HoriuchiOsamu GotohMitsuhide NoshiroYuzo Yoshida
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1998 Volume 124 Issue 4 Pages 694-696

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Abstract

A CYP51-like gene (Z80226) of Mycobacterium tuberculosis was expressed in Escherichia coli. The product exhibited absorption spectra characteristic of P450. The expressed P450 formed a stoichiometric complex with ketoconazole, one of the specific ligands of CYP51. These findings indicate that the CYP51-like gene of M. tuberculosis actually encodes a P450 having active-site environments similar to those of CYP51, confirming the predicted orthologous nature of this gene to eukaryotic CYP51. Although eukaryotic CYP51s are membrane-binding proteins, the expressed product was accumulated only in the soluble fraction of the host cells.

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© The Japanese Biochemical Society
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