The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Nerve Growth Factor Attenuates Endoplasmic Reticulum Stress-Mediated Apoptosis via Suppression of Caspase-12 Activity
Koji ShimokeHisayuki AmanoSoichiro KishiHitoshi UchidaMotoshige KudoToshihiko Ikeuchi
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2004 Volume 135 Issue 3 Pages 439-446

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Abstract

Following endoplasmic reticulum (ER) stress, which occurs via inhibition of the glyc-osylation of newly synthesized proteins, caspase family proteins are activated to promote ER stress-mediated apoptosis. Here we report that nerve growth factor (NGF) suppressed the ER stress-mediated apoptosis in tunicamycin-treated PC12 cells through an extensive decrease of the caspase-3/-9/-12 activity. Detailed analysis of the mechanism underlying the NGF-mediated cell survival revealed that the activities of all seriate caspases were reduced through the phosphatidylinositol 3-kinase (PI3-K) signaling pathway induced by NGF. Moreover, we found that the activity of c-Jun N-terminal kinase (JNK) was not essential for the tunicamycin-induced apoptosis of PC 12 cells. These results demonstrate that the inactivation of caspase-12 via the NGFmediated PI3-K signaling pathway leads to inactivation of the caspase cascade including caspase-3 and -9.

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