1964 Volume 55 Issue 2 Pages 154-162
1. Two distinct hemoglobins were chro-matographically separated from red cells of Anguilla japonica.
2. From the sedimentation and diffusion measurements, the molecular weight was determined to be 65, 200 for one (named E1) of these hemoglobins and to be 68, 800 for the other (named E2)
. 3. The isoelectric point of E1 was to be 8.08 and of E2, 5.96.
4. Absorbancy maxima of the deriva-tives of these hemoglobins are presented.
5. Twelve sulfhydryl groups were found in one mole of E2, while none in E1.
6. One mole of both hemoglobins con-tains four moles of protoheme.
7. Tyrosine and tryptophan contents and finger print maps indicated that these hemoglobins may differ from each other in primary structure.
8. E1 was more resistant than E2 to alkali-denaturation and to autoxidation in the presence of benzoate.
9. As tested with ethyl isocyanide, Et exhibited the same order of the heme-heme interaction with that of horse hemoglobin, but much less interaction in E2, and both hemoglobins completely lacked the Bohr effect.
The authors wish to express their appreciation to Miss M. Takahashi, Department of Physiological Chemistry, Faculty of Pharmaceutical Science, Univer-sity of Tokyo, for the performance of ultracentrifugal analysis.
This work was supported by a Grant for Scien-tific Research from the Ministry of Education.
