Globin prepared from hemoglobin of the European hedgehog (Erinaceus europaeus) was separated into α and β polypeptide chains by chromatography on a CM 52 column. The Saminoethylated α and β chains were each digested with trypsin and the resulting peptides were isolated. The sequences of all the tryptic peptides were established. The ordering of these peptides in the α and β chains was deduced from their homology with the primary structures of α and β chains of human adult hemoglobin. Comparing the primary structures of the α and β chains of adult hemoglobin of the European hedgehog thus obtained with those of adult hemoglobin of the tupai (Tupaia glis), 35 amino acids substitutions in the α chains and 30 in the β chains were recognized.